Earliest: 8–10 DPO (hCG ≥5 mIU/mL)
Accurate: 12–14 DPO or 1 day after missed period
IVF: 9–11 days post-transfer
Pregnancy Test Timing Calculator 2025: When to Test for Accurate Results
Pregnancy tests detect **hCG** — hormone produced after implantation. Our calculator shows **earliest** and **most accurate** test days.
hCG Levels by Day
| DPO | hCG Range (mIU/mL) | Test Sensitivity |
|---|---|---|
| 8 | 5–50 | Early tests (10 mIU) |
| 10 | 25–100 | Most tests (25 mIU) |
| 12 | 50–200 | High accuracy |
| 14 | 100–400 | 99% accurate |
| 16+ | 400–10,000+ | Digital tests |
When to Test
- 8–10 DPO: Earliest (faint line possible)
- 12–14 DPO: Most accurate
- 1 day after missed period: 99% detection
- IVF Day 5: Test 9–11 days post-transfer
Test Types
- Early Response: 10 mIU/mL (6 days before period)
- Standard: 25 mIU/mL (1 day before)
- Digital: 25–50 mIU/mL (clear "Pregnant")
False Negatives
- Testing too early
- Diluted urine (not FMU)
- Low hCG (slow riser)
- Chemical pregnancy
IVF Testing
- Day 3 transfer: Test 12–14 days post
- Day 5 transfer: Test 9–11 days post
- Beta hCG blood test at clinic
Symptoms Before Testing
- Implantation bleeding (6–12 DPO)
- Breast tenderness
- Fatigue, nausea
Pro Tips
- Use first morning urine
- Wait 3 minutes for result
- Retest in 48 hours if negative but period late
- Avoid excessive fluids before test
FAQs
Can I test at night? Yes, but FMU is best.
Evaporation line? Read within 10 min.
Positive then negative? Possible chemical pregnancy — see doctor.
Conclusion
Use our **2025 Pregnancy Test Calculator** to avoid false negatives. Confirm with blood test if unsure.
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